Purpose: This study aims to investigate the effects of rest intervals, sprint distance, and number of repetitions on performance variables, physiological responses, and energy system contributions in repeated-sprint protocols when total distance variable was the controlled (300 m). Method: Sixteen male soccer players participated in this study. The four protocols, each totaling a distance of 300 m, consisted of the combination of 15 and 30 m sprints with 30 s rest intervals (15meters30sec and 30meters30sec, respectively) and 1:5 work-rest ratios (15meters12sec and 30meters22sec, respectively). Aerobic, glycolytic, and phosphagen energy systems` contributions were calculated from the oxygen consumption (VO2) during the exercise, net lactate production, and the recovery VO2 kinetics using mono-exponential models. Repeated measures ANOVA with the Bonferroni correction was applied to examine the hypothesized differences. Results: The findings indicated that total sprint duration (F3:45=281.14; p<0.001), percentage of performance decline (F3:45=16.58; p<0.001), delta lactate (F3:45=39.72, p<0.001), rating of perceived exertion (F3:45=28.64; p<0.001), energy demand (F3:45=101.6; p<0.001), VO2 during the rest intervals (F3:45=42.72; p<0.001), and the absolute contribution of glycolytic (F3:45=119.6; p<0.001) and phosphagen energy systems (F3:45=72.9; p<0.001) were lowest in the 15meters30sec compared to other protocols. However, the relative contribution of aerobic system was greatest in the 15meters30sec compared to other protocols (F3:45=28.1, p<0.001). Both absolute (F3:45=119.6; p<0.001) and relative contribution of glycolytic system (F3:45=88.5, p<0.001) were greatest in the 30meters22sec compared to other protocols. Conclusion: This study showed that increasing sprint distance when rest interval is equal and decreasing rest interval when sprint distance is equal can increase the glycolytic system contribution.
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