Near-infrared spectroscopy is currently used to assess changes in the oxygen saturation of the muscle during exercise. The primary purpose of this study was to assess the reliability of near-infrared spectroscopy in determining muscle oxygen saturation (StO2) in the vastus lateralis during cycling and the gastrocnemius during running for exercise intensities at lactate threshold and maximal effort. Test-retest reliability was determined from an intraclass correlation coefficient obtained from a one-way analysis of variance. Reliability of muscle StO2 for the gastrocnemius at lactate threshold was R = .87, and R = .88 at maximal effort. Reliability of muscle StO2 for the vastus lateralis at lactate threshold was R = .94 and R = .99 at maximal effort.
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