• The dual stress challenge resulted in significant increases in subjective anxiety compared to exercise alone. • The workload reduced post exercise levels of glutathione and advanced oxidation protein products. • The addition of psychological stress during exercise did not result in additional oxidative stress compared to exercise alone. Purpose: Those engaged in high stress occupations such as firefighters and military personnel are exposed to a variety of psychological and physiological stressors. The combination of mental and physical stress [i.e., dual stress challenges (DSC)] results in significant increases in stress hormones, which causes oxidative stress (OS) and contributes to elevated risk for cardiovascular disease. However, data are needed to determine the impact of DSC on markers of OS in exercise-trained individuals. Method: Fourteen healthy trained men aged 21-30 yrs. participated in a randomized, cross-over design to investigate the impact of DSC on blood markers of OS. The exercise alone condition (EA) consisted of 35 min of cycling at 60% VO2 peak. The DSC involved 20 min of mental stress challenges during exercise. Blood was sampled before exercise, as well as immediately, and 30 and 60 min after exercise and analyzed for glutathione (GSH), superoxide dismutase (SOD), hydrogen peroxide (H2O2) and advanced oxidation protein products (AOPP). Result: No significant treatment × time interactions were found. No time effects were noted for SOD, or H2O2; however, AOPP were reduced at 30 (p = .034) and 60 min post exercise (p = .006). GSH was reduced at 30 (p = .009) and 60 min post exercise (p = .031). Conclusion: These results indicate the OS response from DSC is not greater than that produced from EA in exercise trained men. Future work should investigate the impact of chronic resistance and endurance exercise training on OS resulting from DSC.
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