This study aimed to investigate between- and within-team changes in workload [PlayerLoad (PL), training impulse (TRIMP) and session rate of perceived exertion training load (sRPE-TL)], readiness [heart rate variability (HRV)], and physical performance [20-m sprint test (including 10-m split time), countermovement jump (CMJ) and yo-yo intermittent recovery test level 1 (YYIR1)] during 3-week intensified preparation periods in female, national Under18 (n = 12, age = 18.0 ± 0.5y, stature = 180.4 ± 7.5 cm, body mass = 72.7 ± 9.3 kg) and Under20 (n = 12, age = 19.6 ± 0.8y, stature = 178.6 ± 6.4 cm, body mass = 68.0 ± 5.9 kg) basketball teams. Under18 team revealed small-to-moderate statistically significantly higher values in workload [PL: p = 0.010; ES = Small; TRIMP: p = 0.004; ES = Moderate; sRPE-TL: p < 0.001; ES = Moderate] and moderately lower readiness values (p = 0.023; ES = Moderate) compared to Under20. Within-team analysis showed no differences in workload in Under20 and statistically significant reduction (p < 0.05) in Week3 (taper period) in Under18. Pre- and post-preparation changes showed Under18 increasing only YYIR1 performance (p < 0.001; ES = Very large). Differently, Under20 statistically improved in 10-m split time (p = 0.003; ES = Moderate), CMJ (p = 0.025; ES = Moderate) and YYIR1 (p < 0.001; ES = Large). A constant adequate workload positively benefits players` readiness and physical performances during short intensified preparation periods. Conversely, using high workload with periodization strategies encompassing short overload and taper phases induced positive changes on players` aerobic performance, lower readiness values and no changes in anaerobic performances.
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