This study developed and validated a vector magnitude (VM) two-regression model (2RM) for use with an ankle-worn ActiGraph accelerometer. For model development, 181 youth (mean ± SD; age, 12.0 ± 1.5 yr) completed 30 min of supine rest and 2-7 structured activities. For cross-validation, 42 youth (age, 12.6 ± 0.8 yr) completed approximately 2 hr of unstructured physical activity (PA). PA data were collected using an ActiGraph accelerometer, (non-dominant ankle) and the VM was expressed as counts/5-s. Measured energy expenditure (Cosmed K4b2) was converted to youth METs (METy; activity VO2 divided by resting VO2). A coefficient of variation (CV) was calculated for each activity to distinguish continuous walking/running from intermittent activity. The ankle VM sedentary behavior threshold was =10 counts/5-s, and a CV=15 counts/5-s was used to identify walking/running. The ankle VM2RM was within 0.42 METy of measured METy during the unstructured PA (P > 0.05). The ankle VM2RM was within 5.7 min of measured time spent in sedentary, LPA, MPA, and VPA (P > 0.05). Compared to the K4b2, the ankle VM2RM provided similar estimates to measured values during unstructured play and provides a feasible wear location for future studies.
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