Objectives: The aim of our study was to assess the relationship between 25(OH)D levels and muscle stiffness and elasticity in well-trained football players. Material and methods: We enrolled 31 Polish first league football players. The mean: age was 26.3 ± 5.9 years, height was 183.0 ± 7.6 cm and body mass was 78.4 ± 6.9 kg, career duration was 16.7 ± 5.2 years. Serum levels of 25(OH)D were measured by electrochemiluminescence (ECLIA). Muscle stiffness and elasticity was tested with MyotonPRO device. Results: Our study showed decreased serum 25(OH)D levels in 68% of the professional football players. The results also demonstrated a slight statistically significant correlation between vitamin D levels and muscle stiffness in points 12, 22 (located internal hamstrings and gastrocnemius) and elasticity in point 4 (located vastus lateralis). We have found significant positive correlation between muscle stiffness and CK activity. Conclusion: Based on our study we concluded that in well-trained professional football players, there was slight correlation between serum levels of 25(OH)D and muscle stiffness and elasticity measured immediately after the transition period.
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