This study aimed to (a) quantify weekly fluctuations in hormonal responses (testosterone [T], cortisol [C], and their ratio [T:C]), external (PlayerLoad [PL] and PL·minute-1) and internal (session rating of perceived exertion load [sRPE-load], summated heart rate zones [SHRZs], and percentage of maximal heart rate [%HRmax]) load measures, and well-being measured using a self-reported questionnaire and (b) determine the associations between weekly changes in hormonal responses and load measures with weekly changes in well-being during the pre-season phase in basketball players. Twenty-one professional male basketball players (age: 26.2 ± 4.9 years) were monitored during a 5-week pre-season phase. Linear mixed models were used to determine weekly differences in each variable and associations between weekly changes in hormonal and load variables with weekly changes in well-being. Findings revealed that T (p < 0.001) and T:C (p = 0.002) increased toward the end of the pre-season phase. Moreover, higher (p < 0.05) external (PL·minute-1) and internal (%HRmax) load intensities were evident during the first 3 weeks of the pre-season, with no significant fluctuations in other load variables. Weekly changes in PL and sRPE-load were negatively associated (p < 0.05) with weekly changes in well-being, albeit weak in magnitudes (R2 = 0.061-0.105). These results highlight that a periodized approach was undertaken across the pre-season predominantly predicated on altering weekly load intensities, which coincided with positive hormonal responses toward the end of the pre-season. In addition, weak relationships were evident between weekly changes in hormonal responses and load measures with well-being, emphasizing that a low commonality may be present between these constructs.
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