Introduction: The aim of the study was to compare two relay modalities used in cyclist team time trial (TTT) in term of performance, physiological responses and perceived exertion. Facts: A better performance was realized with the one-line-relay-modality compared to the double-line-relay-modality (42.0 vs. 40.5 km/h). The power output (78.5 ± 2.2 vs. 74.5 ± 2.5% MAP) as well as the HR (88.5 ± 2.2 vs. 86.0 ± 1.9% maxHR) average values were significantly higher for TTT-one-line-relay than for TTT-double-line-relay (P < 0.05). The TTT-one-line-relay has been perceived as more difficult (P < 0.05). Conclusion: In terms of performance the TTT-one-line-relay is more efficient but also more difficult than the TTT-double-line-relay. Thus, these two approaches are interesting in competing but need feed different ambitions.
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