This study examined the validity and reliability of a 30-second running sprint test using two non-motorized treadmills compared to the established Wingate Anaerobic Test. Twenty-four participants completed three sessions in a randomized order on a: (1) manual mode treadmill (Woodway); (2) specialized interval training treadmill (HiTrainer); and (3) Wingate cycle ergometer. In a subset of 15 participants, 2 additional sessions were completed on both treadmills to establish the test-retest reliability. Peak (Woodway: r = .68; HiTrainer: r = .58; p < .003), average (Woodway: r = .82; HiTrainer: r = .72, p < .001), and minimum (Woodway: r = .64; HiTrainer: r = .42, p < .043) speed indices were moderately to very strongly correlated with corresponding Wingate Anaerobic Test outputs and had excellent test-retest reliability (all intraclass correlation coefficients > .75). Fatigue index during the Wingate Anaerobic Test (51.20 ± 7.14%) was moderately correlated with the Woodway (32.9 ± 10.9%, r = .55, p = .005) only. This 30-second running sprint test may be a valid and reliable mode-specific alternative to the Wingate Anaerobic Test.
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