Introduction: We express the detection of the prohibited substance prednisone, prednisolone in player`s urine sample by long and intensive use eye steroid drops. Case Presentation: In a rare case in Iranian football, a player`s urine sample had corticosteroids. After all investigations, it was demonstrated that systemic effects can be induced by using long-term of ophthalmic prednisone. Conclusions: It seems to be required to investigate the systemic effects on long term and excessive use of topical corticosteroid drops can have on the result of the sample analysis and showing positive results.
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