Introduction To solve the problem of biomedical parameter in vivo, non-invasive and real-time during exercises, a three-wave-length near-infrared spectroscopy(NIRS) based on diffusion theory of optics was designed[1] for measuring muscle oxygenation content .The system was experimented and tested in various sports events with the purpose of introducing new testing technology and index for monitoring and evaluating biology. Method Twenty healthy volunteers performed on an incremental intensity running protocol. The relative changes of skeletal muscle oxygenation content were measured by NIRS, with which blood lactate acid concentration (BLa), ventilation threshold (VT), heart rate (HR) and Vo2 were compared. Result In the experiment, the change in skeletal muscle oxygenation content of the volunteers was regular and has a significant close relationship to BLa,Vo2, Vco2/ Vo2 and HR (the correlation coefficient of HbO2 and BLa is R=-0.896; HbO2 and VO2 is R=0.958); meanwhile, Vco2/ Vo2 Plateau Duration was consistent with the content of HbO2 descended.
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