This study aimed to examine the acute effects of concurrent muscle strength and sport-specific endurance exercise order on immunological stress responses, metabolic response, muscular-fitness, and rating-of-perceived-exertion (RPE) in highly trained youth female judo athletes. Thirteen female participants randomly performed two concurrent training (CT) sessions; strength-endurance and endurance-strength. Immune response, metabolic response, muscular fitness (i.e., countermovement jump-derived force and power [CMJ-force and CMJ-power]), and RPE were measured at different time points (i.e., PRE, MID, POST, POST6h, and POST22h). There were significant time × order interactions for lymphocytes (p = 0.006, ES = 1.31), granulocyte-lymphocyte ratio (p = 0.002, ES = 1.56), and systemic inflammation index (p = 0.029, ES = 1.11), blood glucose and lactate (p < 0.001, ES = 2.09 and p = 0.0018, ES = 1.51, respectively), CMJ-force (p = 0.033, ES = 1.26), and CMJ-power (p = 0.007, ES = 1.40) as well as RPE (p < 0.001, ES = 2.05). CT-induced acute (i.e., POST) but not delayed (i.e., POST6h and POST22h) order-dependent immune cell count alterations in highly trained youth female judo athletes. All markers of the immune system went back to baseline values at POST22h. Metabolic responses were slightly higher following the endurance exercise (irrespective of the applied exercise order). CMJ-measures and RPE fluctuated during both CT sessions but returned to baseline 6 h post-exercise. Summary - Concurrent strength and sport-specific endurance exercises induced acute (= 15 min) but not delayed (= 6 h) order-dependent immune cell count alterations in highly trained youth female judo athletes. More particularly, the strength-endurance order seems to have led to slightly higher acute immunological activation compared to the endurance-strength order. - All markers of the immune response taken post 22 h returned (or were close) to baseline values, suggesting a sufficient recovery from the exercise-induced immune stress reaction within 22 h in female judo athletes. - Metabolic responses (i.e., lactate, glucose) were slightly higher, following the endurance exercise (irrespective of the applied exercise order).
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