The characteristics of the workload in orienteering presumes a specific dynamic of the functional parameters when applying testing protocols with increase of speed and gradient of the treadmill, compared to the sole increase of the running speed. There is a limited number of publications analyzing the influence of the modifications in the speed and gradient (without changes in the stage length) on the ventilatory threshold (VT) in orienteers. We compared the reproducibility of the most widely used methods for VT determination using protocols with increase of the running speed alone and with modifications of the speed and the gradient. The results demonstrated that the VTEqO2 and VTV-slope - methods were more reproducible for the determination of VT than the VTRER=1.0. Probably, the differing characteristics of the motor activity and direct dependence of the determined VTRER=1.0 on RER = 1.0 - values have an impact on the lower reproducibility of the VTRER=1.0 - method.
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