Background Chronic ankle instability (CAI) is associated with balance disorders and functional deficits in soccer players. Impaired gluteal muscle function contributes significantly to these issues, as these muscles play a crucial role in maintaining lower limb stability and balance. This study aimed to evaluate the effects of Kinesio-taping (KT) on the electromyographic (EMG) activity of gluteal muscles, ankle function, and dynamic balance in male soccer players with CAI. Methods In this randomized clinical trial, 30 male soccer players with CAI were randomly assigned to either the gluteal KT group (case) or the sham control group. Gluteal EMG activity, onset time, ankle function (Single-Leg Hop Test), and dynamic balance (modified Star Excursion Balance Test, mSEBT) were assessed pre-taping, immediately post-taping, and 48 h later. Results The case group demonstrated significant increases in gluteus medius EMG activity (P < 0.05) and reductions in gluteus maximus onset time (P < 0.05) at both immediate and 48-hour follow-ups. Statistically significant improvements were also observed in single-leg hop distance (P = 0.001) and dynamic balance (P < 0.05) in the KT group compared to the control group. However, changes did not consistently meet minimal clinically important difference (MCID) thresholds for the mSEBT (4-5 cm per direction) or single-leg hop test (8-10 cm). Conclusion KT enhances gluteus medius activation, reduces gluteus maximus onset time, and improves ankle function and dynamic balance in soccer players with CAI. While statistically significant, the observed improvements may require integration with other rehabilitation strategies to achieve clinically meaningful outcomes. Future studies should explore long-term effects, diverse populations, and combined interventions to optimize functional gains. Trial registration IRCT2015021015932N10, Date of registration: 04/11/2015.
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